Enabling functional studies of individual cells in plant tissues

18 March 2016 - By: Jim Ruddock

Enabling functional studies of individual cells in plant tissues

By Jim Ruddock

Maria Mar Marquès-Bueno, Ana K. Morao, Anne Cayrel, Matthieu P. Platre, Marie Barberon6, Erwann Caillieux, Vincent Colot, Yvon Jaillais, Françis Roudier and Grégory Vert

The Plant Journal 85(2): 320–333. Article first published online: 21 JAN 2016 | DOI: 10.1111/tpj.13099

Multicellular organisms are composed of many cell types that perform discrete functions acquired via controlled patterns of gene expression and are dictated in part by cell type-specific promoter activity. In order to understand the contribution of these specialized cell types in the development of a whole organism it is necessary to isolate or analyse different cell types separately.  To do this the authors cloned and validated a collection of 30 tissue-specific promoters that were used to generate transgenic lines for cell type-specific induction of gene expression and for cell-type specific nuclei isolation using an optimised INTACT protocol or FACS. In order to demonstrate the power of this tool set, the contribution of each root cell type on IRT1-dependant iron uptake was investigated. This led to new insights showing that IRT1 expression in both the epidermis and phloem companion cells is necessary and sufficient for proper iron homeostasis. The generation and characterisation of this collection of promoters, constructs and transgenic lines represents a huge amount of work. The availability of such tools will contribute to improving our understanding of the role of cell-type specific genome regulation allowing researchers to express or down-regulate their gene of interest in a cell-specific manner. 

Category: Plant Biology
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Jim Ruddock RS

Jim Ruddock

Jim Ruddock is the Editorial Manager for The Plant Journal and Plant Biotechnology Journal, and manages all processes of each journal through the peer review system – from submission to acceptance and export of final files to the Production department. Currently at Wiley, Jim has 23 year’s of experience working in science journal publishing environments in various capacities.