Purification of polyphenol oxidases from fruit

Polyphenol oxidase (PPO) enzyme is of great commercial interest because it is related to the browning of fruits. In this practical spanning two weeks, we will use classical biochemical techniques to extract a PPO enzyme from a banana or apple, perform an assay for PPO activity and protein content, and assess data to discuss reproducibility in science.

 Subject:   Biochemistry, Cell Biology, Plant Biology
Author:   Shane Austin
Date Added:   20/02/2023
Material Type:  
  1. Protocol for experiment for lecturers


  2. Results


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